ACADEMICS

Training




Click Here

Projects




Click Here

TRAINING

PCR & Real time PCR techniques and its applications

    • Basic Microbial techniques and their storage Culturing methods, Microbial isolation techniques, Media optimisation, Storing of Microorganisms
    • Basic Microbial and Molecular techniques Microbial culture techniques, Growth curve analysis, Nucleic acid extraction, Electrophoresis separation, Polymerase chain reaction (PCR), Primer designing and standardisation
    • Microbial Gene Expression studies Microbial nucleic acid extraction and Quantification, Primer designing and standardisation, Real Time PCR: Gene Expression studies, Result Interpretation
    • Human Gene Expression studies Human nucleic acid extraction and Quantification, PCR, Primer designing and standardisation, Result interpretation

Translational Cancer Research Techniques and Molecular Cloning and Cancer Hall-mark research techniques

  • Bacterial Cloning Technique
    • Media preparation and vector selection
    • Bacteria culture and Maintenance
    • Preparation of Competent cells
    • Restriction digestion
    • Ligation and Transformation
    • Small scale isolation of plasmid DNA
    • Large scale isolation of plasmid DNA
    • Restriction digestion
    • Ligation
    • Gel Electrophoresis
    • Elution of DNA from gel
    • Expression studies
  • Proteomics
    • Culture maintenance
    • Extraction and estimation of protein
    • Purification of protein
    • SDS-PAGE
    • Staining
    • Western Blot
    • Western Blot visualization
  • Technical Training
    • Introduction of basic components of Cell culture and environment
    • Media preparation
    • Sterile technique and contamination control
    • Specific cell types and their requirement
    • Culture of cells in attachment and suspension and scaling up of cultures
    • Passaging of cells
    • Study of invitro cell growth behaviour
    • Determination of cell growth
    • Cell viability and cell proliferation assay techniques
    • Cryopreservation and retrieval of cells from frozen storage
  • Functional studies related to Cancer
    • Soft agar assay
    • Clonogenic assay
    • Wound healing assay
    • Proliferation assay
    • Invasion/Migration assay
    • Angiogenesis
  • Functional studies related to Cancer
    • Soft agar assay
    • Clonogenic assay
    • Wound healing assay
    • Proliferation assay
    • Invasion/Migration assay
    • Angiogenesis
  • DNA damage and repair assays
    • DNA damage marker studies by western blot
    • DNA fragmentation
    • Comet assay
    • Pulse field gel electrophoresis
    • Halo assay

    BIO-COMPUTING

    • Drug Designing
    • Protein Structure Prediction
    • Sequence analysis
    • Clinical SAS
    • Nucleotide Database Search
    • Nucleotide Sequence Retrieval
    • Sequence Alignment
    • Primer Designing
    • Phylogenetic Tree Construction
    • Protein Sequence Retrieval
    • RASMOL - Protein Structure Visualization tool
    • sbdbv - Swiss-PdbViewer
    • Motif scan and Identification
    • Hex – Docking
    • ExPASy
    • ISIS Draw – Structure Drawing
    • NCBI Literature Search
  • Special Techniques
    • Cell line revival, culture and storage methods
    • Analysis of gene expression across cell lines by semi-quantitative RT-PCR
    • Analysis of gene expression across cell lines by quantitative RT-PCR
    • Killing curve assay for eukaryotic selection markers
    • Transient gene silencing in cell line by liposome mediated SiRNA transfection
    • Analysis of the impact of gene expression in human cell line
    • Transient exogenous gene expression in human cell line
    • Engineering of human cell line to continuously express the gene on interest
    • Stable gene silencing by shRNA plasmid transfection/transduction
    • Analysis of protein expression in cell lines by Western blotting.
    • Gene over expression in human cells by reverse transfection.
    • Analysis of signalling pathway activity in human cell lines by dual luciferase reporter assay
    • Impact of modulation of gene expression on signalling pathway.
    • Analysis of anchorage independent growth property of human cells.
    • Determination of in-vitro toxicity of a compound on a call line.
    • Investigation of the epigenomic repression of a gene expression in cell line.
    • Analysis of cellular differentiation in human cell line.
    • Elucidation of the transcriptional regulation of gene promoters by reporter assay.
    • Determination of transcription factor-DNA interactions in human cells by Chromatin Immunoprecipitation (ChIP) assay.
    • Investigation of the epigenetic modification of a genomic region by bisulfite methylation assay.
    • Analysis of cellular apoptosis by flow cytometer/Western blot
    • Cell cycle phase analysis of human cell lines by flow cytometer.
    • Investigation of the cellular localization of the protein of interest by immunostaning.

PROJECTS

  • What next after U.G / P.G?
  • Secrets behind Smart work
  • CSIR/UGC/NET/ICMR/ICAR
  • TOEFL / GRE / IELTS Preparation
  • CV/ Resume Preparation
  • Hints to face an Interview